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2 592 680

(13)

(51)

IPC

C12N5/02(2006-01-01)

C12P21/00(2006-01-01)

C12P21/08(2006-01-01)

C07K16/28(2006-01-01)

(21) (22)

Application

2013153090/10, 2012-04-27

(24)

Start date

2012-04-27

(22)

Actual filing date

2012-04-27

(45)

Published

2016-07-27

(72)

Inventor

Srivastava, RuchikaKhemdev, Snekha, LakshmandasBkhatnagar, AnkurDesan, SaravananDzhoel, AnujAjer, KharishRadzha, VanaRao, Lavanya

(73)

Holder

Biokon Riserch Limited

METHOD FOR REDUCTION OF ACCUMULATION OF LACTATE DURING CULTURING AND METHOD OF PRODUCING ANTIBODY Russian patent published in 2016 - IPC C12N5/02 C12P21/00 C12P21/08 C07K16/28

Abstract RU 2592680 C2

FIELD: biotechnology.

SUBSTANCE: to reduce accumulation of lactate from 5 to 40 % of cultivated cells of mammals zinc salt is added in concentration ranging from 0.2 to 0.4 mM. In method for producing antibody zinc salt is added in concentration of 0.2 to 0.4 mM to reduce accumulation of lactate from 5 to 40 % of cultivated cells of mammals, producing above antibody, and antibody is removed.

EFFECT: disclosed is method for reduction of accumulation of lactate from 5 to 40 % when culturing cells and method for producing antibody.

9 cl, 7 dwg, 9 tbl, 7 ex

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RU 2 592 680 C2

Authors

Srivastava, Ruchika

Khemdev, Snekha, Lakshmandas

Bkhatnagar, Ankur

Desan, Saravanan

Dzhoel, Anuj

Ajer, Kharish

Radzha, Vana

Rao, Lavanya

Dates

2016-07-27—Published

2012-04-27—Filed