FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology and specifically to obtaining a protein which is a soluble receptor in a mammalian cell culture, and can be used in medicine to prepare pharmaceutical compositions of a soluble receptor. The method involves: (a) growing mammalian cells in a culture at low temperature ranging from 27.0°C to below 30.0°C; and (b) growing cells in the culture at low pH ranging from 6.8 to less than 7.0. Another version of the method involves: (a) growing said cell culture in a temperature range from 27.0°C to below 30.0°C; wherein the level of glycosylation of the obtained protein increases as temperature rises, or falls as temperature falls to a predetermined level; and (b) growing said cell culture at pH ranging from 6.8 to less than 7.0; wherein the level of glycosylation of the obtained protein increases as pH increases, or falls as pH drops to a predetermined level.
EFFECT: invention enables to obtain a protein product - a soluble receptor, having fewer misfolded and/or aggregated molecules.
23 cl, 45 dwg, 1 tbl, 2 ex
