FIELD: biotechnology.
SUBSTANCE: medium does not contain whey and protein and is characterized by the mole fraction ratio of sodium ions and potassium ions being approximately 10 to 1 to approximately 1 to 1, where the concentration of sodium ions is between approximately 50 and approximately 90 mM and where the mole fraction ratio of ions concentration and total amino acid concentration ranges from approximately 1.9 to approximately 4. A method for production of a recombinant polypeptide comprises mammalian cells culturing in the said medium and expressing a recombinant polypeptide. A method for production of the said medium is also provided, which comprises various components mixing with each other.
EFFECT: high productivity of polypeptides using this medium in mammalian cell culture systems.
13 cl, 6 dwg, 5 tbl, 2 ex
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