METHOD OF PREDICTION OF IDIOPATHIC MALE INFERTILITY BASED ON ANALYSIS OF NUCLEOTIDE VARIANTS IN MITOCHONDRIAL CYTOCHROME B GENE Russian patent published in 2023 - IPC G01N33/58 C12Q1/6806 C12Q1/6827 C12Q1/686 C12Q1/6869 C12Q1/6876 C12Q1/6883 

FIELD: medicine, andrology, reproductive medicine and genetics.

SUBSTANCE: invention can be used to predict idiopathic male infertility. The ejaculate obtained by masturbation is collected, incubated in a thermostat at 37°C, after which mitochondrial DNA is isolated. The genotypes of polymorphic loci in the mitochondrial cytochrome B (MT-CYB) gene are determined using the TaqMan allele discrimination method. If the CC genotype of the rs527236194 polymorphic variant of the MT-CYB gene is detected, the risk of developing idiopathic male infertility in the form of oligoasthenoteratospermia or asthenoteratozoospermia is predicted.

EFFECT: method provides increased accuracy of infertility prediction by expanding the types of pathologies to be detected: oligoastenoteratospermia and asthenoteratozoospermia.

1 cl, 1 tbl, 3 ex