FIELD: biotechnologies.
SUBSTANCE: claimed invention discloses the attenuation of post-translational fucosylation of the molecule containing a modified Fc-section. The claimed process comprises the Fc-section modification, molecule-candidate expression, evaluation of the ratio between the glycosylation of oligosaccharides with the high content of mannose and the glycosylation of complex oligosaccharides and identification of the molecule-candidate as a suitable one at the ratio over 0.2. The proposed invention can be used in medicine.
EFFECT: higher efficiency of purification.
15 cl, 95 dwg, 35 tbl, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| COMBINED THERAPY FOR CANCER TREATMENT | 2016 |
|
RU2731202C2 |
| TRISPECIFIC BINDING MOLECULES, WHICH SPECIFICALLY BIND ANTIGENS OF A VARIETY OF MALIGNANT TUMORS, AND METHODS OF USING THEM | 2015 |
|
RU2718692C2 |
| COMBINED THERAPY FOR MALIGNANT TUMOR TREATMENT | 2016 |
|
RU2733315C2 |
| DERIVATIVES OF PROTEIN FUSED WITH Fc WITH HIGH DOUBLE ACTIVITY: ANTIVIRAL ACTIVITY RELATIVELY TO HIV AND IMMUNOMODULATING ACTIVITY | 2018 |
|
RU2774782C2 |
| IMPROVED METHODS OF TREATING VASCULARISED MALIGNANT TUMORS | 2015 |
|
RU2692248C2 |
| OPTIMIZED Fc-VERSIONS THAT HAVE ALTERED BINDING TO FcγR AND METHODS FOR THEIR PRODUCTION | 2004 |
|
RU2337107C2 |
| ANTIBODY Fc VARIANTS | 2012 |
|
RU2607014C2 |
| DESIGN OF STABLE HETERODIMERIC ANTIBODY WITH MUTATIONS IN Fc DOMAIN | 2011 |
|
RU2604490C2 |
| METHOD FOR PREPARING ANTIBODIES HAVING IMPROVED PROPERTIES | 2011 |
|
RU2604811C2 |
| HETERODIMERISED POLYPEPTIDE | 2012 |
|
RU2719132C2 |
