METHOD OF IN VITRO SELECTION OF ACID-RESISTANT FORMS OF CLOVER Russian patent published in 2016 - IPC A01H1/04 

FIELD: agriculture.

SUBSTANCE: invention is a method for in vitro selection of acid red clover genotypes comprising culturing morphogenic clover culture on a nutrient medium in Gamborg B₅, Where morphogens culture obtained by germination and cultivation of seedlings produced on a nutrient agar medium in Gamborg B₅ with 2.0 mg/L 6-benzylaminopurine, and 100 mg/l Al³⁺ when subculturing on a medium of the same composition, but without the addition of Al³⁺ epicotyls of seedlings, roots have formed at least 4-5 mm on a selective medium with 100 mg/l Al³⁺, at the same time as acid resistant plants are selected clover plants with long roots of at least 50 mm.

EFFECT: invention can be used in plant breeding to create the raw material of a clover meadow with increased resistance to soil acidity, in research on the physiology and genetics of plants.

1 cl, 1 dwg, 3 tbl, 2 ex