METHOD OF PRODUCING MICROENCAPSULATED FORM OF THERAPEUTIC PROTEIN SUPEROXIDE DISMUTASE FOR ORAL APPLICATION Russian patent published in 2016 - IPC A61K9/50 

FIELD: chemistry.

SUBSTANCE: invention relates to high-molecular chemistry and pharmacology, and is intended for use as an oral form of therapeutic protein superoxide dismutase (SOD). Invention consists in adding SOD into porous calcium carbonate (CaCO₃) of core by coprecipitation of solutions Na₂CO₃ and CaCl₂×2H₂O and further formation of alginate microcapsules, containing said core. For suppressing peptidase activity in intestinal medium in system includes peptidase inhibitor - ovomucoid (OM), which, as well as SOD, but independently from it, is included in core. CaCO₃ cores are generated according to following procedure: equal volumes of 0.33 m of solutions Na₂CO₃ and CaCl₂×2H₂O is drained and mixed with 30 s. Suspension matures in 15 minutes, then core is washed with water and acetone, filtered and dried at T = 40 °C. Formation of alginate microcapsules, containing a core with proteins, carried out by ionotropic cross-linking. Dimensions of alginate microcapsules is 800-900 mcm. Inclusion of SOD and OM therein is 10-30 mcg/mg and 5-20 mcg/mg, respectively. Such a configuration of oral delivery system maintains activity of SOD in presence of digestive enzyme trypsin. Release of SOD in medium simulating gastric (0.05 m HCl pH = 1.2) is minimum.

EFFECT: in a medium simulating intestinal (phosphate buffer, pH 8), SOD is released on 60 % for 24 hours that provides prolonged action of preparation.

1 cl, 2 dwg, 2 ex