FIELD: medicine.
SUBSTANCE: invention refers to biotechnology and genetic engineering. DNA is delivered to macroorganism in biocompatible and biodegradable microcapsules made of multilayer polymeric DEAE-dextrane/k-carrageenan membranes protecting DNA from nuclease splitting while delivered to organism cells. Plasmid DNA is sorbed with porous particles CaCO3 by coprecipitating process. The multilayer membranes are produced by sequential layer of DEAE-dextrane and k-carrageenan on the porous particles CaCO3. The microcapsules are placed in 0.1 M EDTA solution. It is agitated at an intensive rate to dissolve an internal core containing CaCO3. The produced microcapsules are washed with water for three times. They are injected in animal cells.
EFFECT: method increases an amount of sorbed DNA to 96,0-99,1 % and provides greater strength of the microcapsule membrane.
3 tbl, 2 ex
| Title | Year | Author | Number |
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| METHOD OF DNA DELIVERY TO MACROORGANISM FOR VACCINES AND SOMATIC GENETIC THERAPY | 2006 |
|
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| VACCINE AGAINST VIRAL INFECTIONS | 2000 |
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| STRAIN OF HYBRID CULTURED MURINE CELLS MUS MUSCULUS L USED FOR PREPARING MONOCLONAL ANTIBODIES TO DIGOXIN | 1993 |
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| STRAIN OF HYBRID CULTURED MURINE CELLS MUS MUSCULUS L USED FOR PREPARING MONOCLONAL ANTIBODIES TO THYROXINE | 1993 |
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| METHOD OF PRODUCTION OF MONOCLONAL ANTIBODIES TO PROTEIN P30 OF AFRICAN SWINE FEVER VIRUS USING RECOMBINANT CONSTRUCTIONS | 2013 |
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