METHOD FOR DETECTION OF GENETIC PREDISPOSITION TO DISRUPTION OF SKIN BARRIER FUNCTION Russian patent published in 2016 - IPC G01N33/50 

FIELD: medicine.

SUBSTANCE: invention relates to method for detection of genetic predisposition to disruption of skin barrier function. Method summary consists in fact that genetic material is recovered from venous blood. DNA synthesis polymerase chain reaction is carried out followed by analysis of polymorphism of restriction fragment lengths, determination of nucleotide sequence and determination of filaggrin 2282del4 polymorphic variant. When carrying out polymerase chain reaction using specific primers: “FLG-2 F1” 5'Biotin-TgA-CCA-gCC-TgT-CCA-Tgg-C3'; “FLG-2 R1” 5'CAA-gTg-CAg-gAg-AAA-gAC-ATg-gAT3', amplicon size is 205 base pairs, and after carrying out polymerase chain reaction pyrosequencing reaction is carried out in real time using specific primer “FLG-2 S1” 5'gAC-ATT-CAg-AAg-ACT-CAg-AC3'. Nucleotide sequence detecting is performed by chemiluminescent signal, it is followed by comparing obtained nucleotide sequence in position 2282 with reference sequences. If obtained pyrogram shows absence of deletion in position 2282 of filaggrin gene at action on body of harmful production factors of irritant and sensitising action low hereditary predisposition caused skin barrier function is defined. If obtained pyrogram shows heterozygous version ins/del 2282 of filaggrin gene high inherited predisposition caused skin barrier function is defined.

EFFECT: using declared method allows to increase efficiency of detection of genetic predisposition to disruption of skin barrier function at action of irritant and sensitising factors action on body.

1 cl, 2 dwg, 1 tbl, 2 ex