METHOD OF PREDICTING RISK OF DEVELOPMENT OF OCCUPATIONAL BRONCHOPULMONARY PATHOLOGY Russian patent published in 2012 - IPC A61B10/00 G01N33/48 

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine. In order to predict risk of development of occupational bronchopulmonary pathology, venous blood is sampled, genetic material is isolated, polymerase chain reaction is performed with specific primers, nucleotide sequence is determined and on the basis of this polymorphic versions of gene of matrix metalloproteinase 1 polymorphism 1607 delG are determined. In carrying out polymerase chain reaction specific primers MP1-F 138F BIOTIN - GCG TCA AGA CTG ATA TCT TAC TC A TAA ACA ATA and MP1-R 138R ACA TgT TAT gCC ACT TAg ATg Agg AAA are used. After carrying out polymerase chain reaction, reaction of pyrosequencing with application of specific primer MP1-S 138S gTA gTT AAA TAA TTA gAA Ag and detection of nucleotide sequence are performed. After that, comparison of obtained nucleotide sequence with reference sequences is carried out and obtained program is used to determine version of polymorphism 1607delG in nucleotide sequence, by which risk of development of occupational bronchopulmonary pathology is predicted.

EFFECT: method increases rate of carrying out prediction of risk of development of occupational bronchopulmonary pathology.

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