FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to an annealed hybrid of a transposon/polynucleotide connection, containing the first transposon, the second transposon, and connection polynucleotide. A transposome system is also disclosed, containing transposase, the first transposon, the second transposon, and connection polynucleotide, as well as a kit containing it.
EFFECT: invention is effective for creation of a library of marked fragments of nucleic acids.
52 cl, 10 dwg, 2 tbl, 9 ex
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