FIELD: chemistry.
SUBSTANCE: presented variants of tetrapyrrol compound production and bacterial cell for its production. Method envisages cultivation of Escherichia coli bacterial cells in culture medium to produce target compound from culture medium. At that, said compound the structure of phthalocyanine ring carrying 4 methyl group and ethyl ester group 4 or propionate groups on porphyrin ring. E. coli is selected from group consisting of e.coli K12, e.coli BL21, e.coli JD23504, which are able to express the gene ypjD (b2611) as result of transposon ypjD gene (b2611) insert into gene of e.coli cell. When implementing versions of method culture medium is used containing elementary Mn, Au, Cu, Zn or Ru able to be transformed into appropriate ions in culture medium, or Mn-containing, Au-containing Cu-containing Zn-containing Ru-containing compound capable of dissociating on corresponding ions in culture medium. Formed as a result of collecting or release from culture medium tetrapyrrol compound has structure of phthalocyanine ring in centre of which coordinated Mn, Au, Cu, Zn or Ru.
EFFECT: invention enables to obtain tetrapyrrol connection without using its precursor.
4 cl, 22 dwg, 6 ex
Authors
Dates
2016-06-27—Published
2008-12-01—Filed