FIELD: biotechnology.
SUBSTANCE: group of inventions relates to genetically modified cells for production of fucosylated oligosaccharide, a method for its preparation and a method for producing fucosylated oligosaccharides. Genetically modified cell is prepared by transforming a cell with a fucose kinase coding gene, a fucose-1-phosphate guanylyltransferase coding gene and a fucosyltransferase coding gene. Catabolic pathway for fucose in said cell is inactivated by inactivating one or more genes selected from a group consisting of a fucose-1-phosphate aldolase gene, a fucose isomerase gene and a fuculose kinase gene. Cell is a microorganism selected from a group consisting of genera Escherichia, Klebsiella, Helicobacter, Bacillus, Lactobacillus, Streptococcus, Lactococcus, Pichia, Saccharomyces and Kluyveromyces.
EFFECT: said genetically modified cell is used to obtain by fucosylated oligosaccharides via cultivation thereof under suitable conditions in a medium which includes fucose and an acceptor substrate, wherein acceptor substrate is a mono-, di-, or oligosaccharide or peptide.
9 cl, 12 dwg, 3 tbl, 11 ex
| Title | Year | Author | Number |
|---|---|---|---|
| HMO SYNTHESIS | 2009 |
|
RU2517602C2 |
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|
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| NEW FUCOSYLTRANSFERASES AND APPLICATIONS THEREOF | 2011 |
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| PRODUCTION OF FUCOSYLATED OLIGOSACCHARIDES IN BACILLUS | 2020 |
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| IMPROVED METHOD FOR PRODUCTION OF FUCOSYLATED OLIGOSACCHARIDES | 2017 |
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| ENZYMATIC METHOD FOR PRODUCING MONOSACCHARIDES IN A FREE FORM FROM SUGARS ACTIVATED WITH NUCLEOTIDES | 2016 |
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| ENZYMATIC PRODUCTION OF CARBOHYDRATES BY MICROBIAL CELLS USING MIXED RAW MATERIALS | 2020 |
|
RU2809122C2 |
| USE OF GLYCOSIDASES IN OLIGOSACHARIDE PRODUCTION | 2019 |
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RU2810730C2 |
| ENZYMATIC PRODUCTION OF OLIGOSACCHARIDES THROUGH GENERAL FERMENTATION USING MIXED RAW MATERIALS | 2019 |
|
RU2801231C2 |
