FIELD: biochemistry.
SUBSTANCE: group of inventions relates to biochemistry. Described is kit of reagents for DNA detection of Neisseria gonorrhoeae in sample, including at least two oligonucleotide primers for polymerase chain reaction and, if necessary, oligonucleotide probe, thermally stable DNA polymerase, buffer solution for PCR, deoxynucleoside triphosphates, characterised by that first oligonucleotide primer has nucleotide sequence SEQ ID NO: 1, second oligonucleotide primer has nucleotide sequence SEQ ID NO: 2. Also described is method of detecting DNA of Neisseria gonorrhoeae in sample by polymerase chain reaction, characterised by that detection is carried out using above set of reagents. Invention can be used in laboratory Diagnostics for DNA detection of Neisseria gonorrhoeae in biological samples by polymerase chain reaction.
EFFECT: group of inventions provides high sensitivity and specificity detection of all clinically significant strains Neisseria gonorrhoeae by means of single target polymerase chain reaction.
8 cl, 4 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| REAGENT KIT FOR CHLAMYDIA TRACHOMATIS DNA DETECTION AND ITS APPLICATION | 2014 |
|
RU2621863C2 |
| NUCLEIC ACID PROBE | 2014 |
|
RU2668154C2 |
| METHOD OF IDENTIFICATION OF SINGLE-NUCLEOTIDE POLYMORPHISMS IN MULTICOPY GENOME LOCUS USING LOOP-POLYMERASE CHAIN REACTION METHOD WITH SUBSEQUENT HYBRIDIZATION ON BIOLOGICAL MICROCHIP | 2022 |
|
RU2809184C1 |
| OLIGONUCLEOTIDE BIOCHIP FOR IDENTIFICATION OF GENETIC DETERMINANT OF NEISSERIA GONORRHOEAE RESISTANCE TO ANTIMICROBIAL PREPARATIONS, SET OF OLIGONUCLEOTIDES USED FOR IMMOBILISATION ON BIOCHIP | 2016 |
|
RU2636457C2 |
| METHOD FOR GENOTYPING NEISSERIA GONORRHOEAE ISOLATES ON BIOLOGICAL MICROCHIP | 2023 |
|
RU2816767C1 |
| SET OF SYNTHETIC OLIGONUCLEOTIDS FOR QUANTITATIVE DETERMINATION DNA UREAPLASMA PARVUM, UREAPLASMA UREALYTICUM AND MYCOPLASMA HOMINIS IN THE VAGINAL MUCOSA | 2017 |
|
RU2663453C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS FOR DETECTING VARIANT STRAINS BURKHOLDERIA THAILANDENSIS, CONTAINING CLUSTER OF CAPSULAR POLYSACCHARIDE BIOSYNTHESIS GENES, HIGHLY HOMOLOGOUS BURKHOLDERIA PSEUDOMALLEI | 2019 |
|
RU2728356C1 |
| METHOD FOR IDENTIFYING MUTATIONS IN THE GENE OF PENICILLIN-BINDING PROTEIN 2 PENA NEISSERIA GONORRHOEAE, LEADING TO RESISTANCE TO BETA-LACTAM ANTIBIOTICS, ON BIOLOGICAL MICROCHIPS | 2020 |
|
RU2741099C1 |
| METHOD OF MICROORGANISM DETECTION IN BIOLOGICAL SAMPLE | 2007 |
|
RU2435865C2 |
| METHOD FOR TARGET AMPLIFICATION OF HUMAN REPRODUCTIVE ORGANS INFECTORS GENOMES FOR SIMULTANEOUS IDENTIFICATION OF INFECTORS WITH PRIMER SET | 2015 |
|
RU2625006C1 |
