FIELD: biotechnology.
SUBSTANCE: invention includes novel probes for isothermal amplification of a nucleic acid and methods for detecting a target nucleic acid sequence using these probes. Said probes comprise an oligonucleotide probe sequence complementary to the region of the target nucleic acid sequence. Said oligonucleotide probe sequence has only one fluorophore label associated with the internal cytosine base, and does not have a 3'-end terminator. Above cytosine base is centrally located along the length of the oligonucleotide, except for positions 1–3 at 3'-end and position 1 at 5'-end.
EFFECT: these probes can be used to detect chlamydia and gonorrhea infections in a patient.
21 cl, 12 dwg, 1 tbl, 12 ex
