FIELD: chemistry.
SUBSTANCE: group of inventions relates to materials and methods of conjugation polysialic acid (PSA) containing active amino oxy group, with oxidized carbohydrate fragments of protein coagulation, including contact oxidised carbohydrate fragment with an activated PSA in conditions which enable to conjugation. Conjugation is carried out by incubation in buffer containing oxidant, selected from group consisting of sodium periodate (NaIO4), lead tetra acetate (Pb (OAc)4) and potassium perruthenate (KRuO4).
EFFECT: obtained using said method conjugates have high activity.
16 cl, 8 dwg, 3 tbl, 28 ex
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|
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| GLYCOPOLYSIALYLATION OF PROTEINS THAT ARE NOT THE PROTEINS OF THE BLOOD CLOTTING | 2014 |
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|
RU2744592C1 |
| N-TAILED POLYSIALYLATION | 2007 |
|
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| CONJUGATED FACTOR VIII MOLECULES | 2009 |
|
RU2573587C2 |
| CONJUGATE OF FACTOR VII POLYPEPTIDE, METHOD OF OBTAINING IT, ITS APPLICATION AND PHARMACEUTICAL COMPOSITION CONTAINING IT | 2003 |
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RU2362807C2 |
| COMPOSITIONS OF RECOMBINANT FURINE | 2012 |
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RU2610436C2 |
| METHOD OF CONJUGATING PEPTIDES, MEDIATED BY TRANSGLUTAMINASE | 2005 |
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RU2385879C2 |
