IMPLANTED MATRIX MATERIAL FOR REGENERATIVE MEDICINE AND METHOD OF ITS PRODUCTION (VERSIONS) Russian patent published in 2016 - IPC C08J3/07 C08J3/75 C08B37/06 C08L5/00 A61K38/39 A61K47/36 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, particularly to a new matrix material for tissue bioengineering and regenerative medicine and methods of its production. Matrix material is designed based on vegetal polysaccharide - pectin with etherification degree of not more than 50% and proteins of intracellular matrix-collagens I and IV types, which contains said components in the following concentrations: 0.5-2.0 wt%, 0.1-1.5 wt% and 0.01-0.5 wt%, respectively. Method of production of the implanted matrix material for regenerative medicine in the form of hydrogel involves mixing at a temperature of 5 °C max of calcium acetate solution of type I collagen to final concentration of 0.1-1.5 wt% with type IV collagen solution to the final concentration 0.01-0.1 wt%, then the produced mixture is mixed with previously prepared gel formation initiator, including calcium chloride solution in the concentration, providing a gel forming process, non-toxic buffer system in amount sufficient for neutralisation of the mixture, and sodium chloride solution up to physiological concentration; then the pectin solution is introduced with degree of esterification of 50% max to the final concentration 0.5-2.0 wt% and the gel is formed by temperature rise up to physiological. Invention also relates to a method of producing implanted matrix material for regenerative medicine in the form of hydrogel, which involves mixing at a temperature of 5 °C max of calcium acetate solution of type I collagen to final concentration of 0.1-1.5 wt% with IV type collagen solution to the final concentration 0.01-0.1 wt%, then the produced mixture is neutralised, stabilised by the non-toxic buffer system and the sodium chloride is added up to physiological concentration then the pectin solution with etherification degree of not more than 50 % to the final concentration 0.5-2.0 wt% is added, to obtain a fluid composition, from which the gel is formed.

EFFECT: invention enables to obtain a biocompatible implantable materials with a low rate of biodegradation and ability to stimulate regenerative process, in particular, recovery of nerve conductors after a brain injury.

10 cl, 8 dwg