METHOD AND PRODUCT FOR LOCALISED OR SPATIAL DETECTION OF NUCLEIC ACID IN TISSUE SAMPLE Russian patent published in 2016 - IPC C12Q1/68 

FIELD: biochemistry.

SUBSTANCE: present invention relates to methods and products for localised or spatial detection of nucleic acid in tissue sample and, in particular, to a method of localised detection of nucleic acid in a tissue sample, involving: (a) providing a chip comprising a substrate on which directly or indirectly are immobilised multiple types of capturing probes, so that each type occupies a certain position on chip and is oriented so that it has a free 3′-end, which enables said probe to act as a primer in an extension reaction or ligation of primer, where each type of said capturing probe contains a nucleic acid molecule, having from 5′ to 3′: (1) position area, which corresponds to position of capturing probe on chip, and (2) capturing area; (b) bringing said chip into contact with tissue sample so that position of capturing probe on chip can be mapped to positions in tissue sample, and enabling nucleic acid of tissue sample to be hybridised with capturing area in said capturing probes; (c) synthesis of DNA molecules based on captured nucleic acid molecules using said capturing probes as primers for elongation or ligation, wherein said elongated or ligated DNA molecules are labelled due to position area; (d) optionally synthesis of complementary thread of said labelled DNA and/or optionally amplification of said labelled DNA; (e) release of at least part of labelled DNA molecules and/or complements thereof or amplicons from surface of chip, wherein said part includes position area or complement thereof; and (f) direct or indirect analysis of sequence of DNA molecules released.

EFFECT: methods and products for localised or spatial detection of nucleic acid in tissue sample.

55 cl, 31 dwg, 2 tbl, 12 ex