FIELD: biotechnology.
SUBSTANCE: invention represents a method based on applying patterns of oligonucleotide probes with barcodes on predetermined areas in a region of interest in a tissue sample. Each analyzed nucleic acid can be localized in a specific position within the sample taking into account a barcode. Various printing techniques can be used and various methods for obtaining patterns similar to a regular matrix with a specific pitch can be used, or alternatively object-oriented generation of patterns using certain regions of interest without limitations in form.
EFFECT: invention enables spatial mapping of nucleic acids of high-resolution tissue samples without sacrificing the degree of multiplexing available when sequencing the next generation.
13 cl, 3 dwg, 3 tbl, 1 ex
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