FIELD: biotechnology.
SUBSTANCE: invention represents a method based on applying patterns of oligonucleotide probes with barcodes on predetermined areas in a region of interest in a tissue sample. Each analyzed nucleic acid can be localized in a specific position within the sample taking into account a barcode. Various printing techniques can be used and various methods for obtaining patterns similar to a regular matrix with a specific pitch can be used, or alternatively object-oriented generation of patterns using certain regions of interest without limitations in form.
EFFECT: invention enables spatial mapping of nucleic acids of high-resolution tissue samples without sacrificing the degree of multiplexing available when sequencing the next generation.
13 cl, 3 dwg, 3 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD AND PRODUCT FOR LOCALISED OR SPATIAL DETECTION OF NUCLEIC ACID IN TISSUE SAMPLE | 2012 |
|
RU2603074C2 |
| PANEL AND METHOD OF OBTAINING SPATIAL INFORMATION ABOUT NUCLEIC ACIDS | 2020 |
|
RU2803202C2 |
| DIRECT CAPTURE, AMPLIFICATION AND SEQUENCING OF TARGET DNA USING IMMOBILISED PRIMERS | 2011 |
|
RU2565550C2 |
| IMPROVED DETECTION OF SHORT HOMOPOLYMERIC REPEATS | 2016 |
|
RU2730622C2 |
| TRANSPOSITION WITH PRESERVATION OF GENE ADHESION | 2015 |
|
RU2736728C2 |
| METHOD AND COMPOSITION FOR ANALYSIS OF CELLULAR COMPONENTS | 2016 |
|
RU2761432C2 |
| GENETIC POLYMORPHISMS IN AGE-RELATED MACULAR DEGENERATION | 2010 |
|
RU2577726C2 |
| GENETIC POLYMORPHISMS ACCOMPANYING AGE-RELATED MACULAR DEGENERATION | 2010 |
|
RU2546008C2 |
| MULTIPLEX DETECTION OF NUCLEIC ACIDS | 2014 |
|
RU2733887C2 |
| METHOD FOR DETECTING HUMAN PARVOVIRUS B19 IN BIOLOGICAL SAMPLE (VARIANTS) AND SET FOR ITS REALIZATION | 2002 |
|
RU2301263C2 |
