CONJUGATION OF STAPHYLOCOCCUS AUREUS TYPE 5 AND TYPE 8 CAPSULAR POLYSACCHARIDES Russian patent published in 2016 - IPC A61K47/48 A61K39/85 A61K39/385 C07H3/00 

FIELD: biochemistry.

SUBSTANCE: present invention relates to biochemistry, particularly to a method of producing a conjugate of S.aureus type 5 or type 8 capsular polysaccharide and a carrier protein. Method involves a step for depolymerisation of capsular polysaccharide by acid hydrolysis to obtain polysaccharide fragment, step for oxidising fragment for introduction of an aldehyde group into at least one saccharide residue in fragment to produce an oxidised saccharide residue and a step of binding oxidised saccharide residue with carrier protein through an aldehyde group. At oxidation step two vicinal hydroxyl groups of polysaccharide are converted to aldehyde. Present invention provides a conjugate of S.aurens type 5 or type 8 capsular polysaccharide and carrier protein, obtained using said method, and an immunogenic composition including said conjugate.

EFFECT: present invention improves immunological properties of obtained conjugate.

13 cl, 18 dwg, 8 tbl, 1 ex