FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and can be used for production of plasminogen and plasmin variants. Version of plasminogen is obtained, containing activation site and catalytic domain, in which catalytic domain contains valine, substituted for isoleucine, in position 1 of catalytic domain of human plasmin or in position, corresponding to such in catalytic domain of plasmin, which differs from human plasmin where said catalytic domain of human plasmin starts with amino acid valine in position 1, which is the same amino acid valine in position 562 Glu-plasminogen of human with SEQ ID NO: 1.
EFFECT: invention enables to obtain autoproteolytically stable variant of plasmin.
19 cl, 14 dwg, 3 tbl, 2 ex
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|
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