PLASMINOGEN AND PLASMIN VARIANTS Russian patent published in 2016 - IPC C07K14/47 C12N9/68 C12N15/11 C12N5/10 A61K38/16 A61K38/48 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and can be used for production of plasminogen and plasmin variants. Method comprises obtaining a variant of plasminogen, containing in its catalytic domain a replacement of glutamic acid with glutamine in position 138 of catalytic domain of human plasmin or in a position corresponding to such in a catalytic domain of plasmin other than human plasmin, where said catalytic domain of human plasmin starts with amino acid valine in position 1, which is same amino acid valine, occurring in position 562 of human Glu-plasminogen with SEQ ID NO: 1. wherein said variant of plasminogen can also contain a replacement of lysine with glutamic acid or histidine in position 147 and/or replacement of arginine with histidine in position 158 of catalytic domain of human plasmin.

EFFECT: invention enables to obtain autoproteolytically stable variant of plasmin.

33 cl, 14 dwg, 2 tbl, 2 ex