FIELD: biotechnology.
SUBSTANCE: present invention relates to a method for capturing virus-like particles of interest from a mixture containing destroyed plant cells. Method comprises use of an expanded bed of adsorbent containing resin material, balancing resin material at pH 6.0–8.0 and adding mixture to expanded bed of adsorbent for binding of virus-like particles. Degree of expansion of expanded bed is equal to 1–5. Further, adsorbent is washed. Virus-like particles are eluted from adsorbent.
EFFECT: high purity of extracted particles, high process efficiency.
17 cl, 9 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| FUSION PROTEIN PURIFICATION METHOD | 2015 |
|
RU2698654C2 |
| PRODUCTION OF RABIES VIRUS-LIKE PARTICLE IN PLANTS | 2012 |
|
RU2655433C2 |
| PRODUCTION OF VIRUS-LIKE PARTICLES IN PLANTS | 2011 |
|
RU2655431C2 |
| VIRUS-LIKE PARTICLES (VLP) OF RABIES VIRUS GLYCOPROTEIN | 2011 |
|
RU2575800C2 |
| OBTAINING PICORNAVIRUS-LIKE PARTICLES IN PLANTS | 2013 |
|
RU2693431C2 |
| COMPOSITION FOR IMMUNIZATION (VARIANTS), METHOD FOR ITS PREPARING AND USING FOR TREATMENT OF ALLERGIC EOSINOPHILIC DISEASES | 2002 |
|
RU2319503C2 |
| OPTIMISED EXPRESSION OF HPV 58 L1 IN YEAST | 2004 |
|
RU2370538C2 |
| PRECIPITATING AND PURIFYING PROTEINS WITH POLYELECTROLYTES | 2008 |
|
RU2474585C2 |
| ANTIGEN MATRIX FOR TREATMENT OF BONE DISEASE | 2002 |
|
RU2322258C2 |
| METHOD OF PRODUCTION OF VIRUS-LIKE PARTICLES OF PLANTS | 2010 |
|
RU2579903C2 |
