FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry. Described is a polypeptide, comprising a Fc-domain of an antibody with at least one amino acid modification, which has high activity of binding with FcγRIIb in comparison with initial polypeptide, where value [value KD polypeptide containing Fc-domain of antibody against FcγRIIa (type R)]/[value KD polypeptide containing Fc-domain of antibody against FcγRIIb] is equal to or greater than 1.2, where value [value KD for higher activity of binding polypeptide containing Fc-domain of antibody against FcγRIIa (type R) and FcγRIIa (type H)]/[value KD for higher activity of binding initial polypeptide on FcγRIIa (type R) and FcγRIIa (type H)] is 0.7 or more, and where amino acid modification is a replacement of Pro in position 238 (EU numeration) with Asp or replacement of Leu in position 328 (EU numeration) with Glu. Described are methods of producing said polypeptide and use thereof.
EFFECT: invention widens range of therapeutic and preventive agents.
20 cl, 16 dwg, 12 tbl, 9 ex
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