METHOD FOR OBTAINING ERYTHROCYTE ANTIGEN FOR DIAGNOSTICS OF NECTOBACTERIOSIS IN ANIMALS Russian patent published in 2017 - IPC G01N33/531 A61K39/114 C12P21/00 

FIELD: medicine, pharmacology.

SUBSTANCE: antigen fraction is obtained by cultivation of industrial strain Fusobacterium necrophorum "0-1" VIEV with further separation of bacterial mass and resuspension with 0.8-1.2% dezmol solution to concentration 4-10 bil/ml of microbial bodies, after that, heated at temperature 93-98°C for 55-65 min, and mixture is centrifuged at 1.5-3.0 thousand g, for 20-30 min. Formalin in final concentration 0.3-0.4% is added to supernatant and incubated at room temperature for 10-15 days. After that, formalised erythrocytes, taken in final concentration 9-12%, are added to reaction mixture, and target product is obtained by incubation of reaction mixture with formalised erythrocytes at 40-46°C for 1.5-2 hours with their further cooling to room temperature and washing.

EFFECT: obtaining erythrocyte antigen for diagnostics of necrobacteriosis in animals makes it possible to increase storage term of target product with preservation of its specific activity.

4 cl, 7 ex