FIELD: chemistry.
SUBSTANCE: method comprises the following steps: (a) diafiltration of the partially purified IgG solution to achieve an ethanol concentration of less than 0.5% (w/v); b) stabilization of the solution produced in step a) using sorbitol as a stabilizing agent at a sorbitol concentration of between 30 wt % and 35 wt %; c) heat treatment of the solution produced in step b) at a temperature between 55°C and 63°C for a period of time between 1 and 24 hours; d) selective adsorbtion of aggregates and/or high molecular weight polymers from the solution subjected to the heat treatment in step c) by cation chromatography; and e) diafiltration and preparation of the solution produced in step d).
EFFECT: invention provides stability of the final product.
27 cl, 22 tbl, 18 ex
| Title | Year | Author | Number |
|---|---|---|---|
| IMPROVING IMMUNOGLOBULIN AFFINITY CHROMATOGRAPHY BY THE APPLICATION OF PRE-CAPTURE FLOCULATION | 2020 |
|
RU2794431C1 |
| CLOSED CONTAINER, CONTAINING ACTIVATED FACTOR VII POLYPEPTIDE METHODS OF OBTAINING IT AND SET AND METHOD OF SET APPLICATION | 2006 |
|
RU2440810C2 |
| METHOD OF AFFINITY CHROMATOGRAPHY TO OBTAIN PURIFIED PROTEIN | 2012 |
|
RU2609633C2 |
| METHODS OF PURIFYING POLYPEPTIDES | 2011 |
|
RU2594163C2 |
| PROTEIN PURIFICATION USING BIS-TRIS BUFFER | 2012 |
|
RU2603347C2 |
| METHOD FOR PURIFYING CONJUGATES BASED ON IL-15/IL-15Rα | 2015 |
|
RU2701301C2 |
| METHOD OF PURIFYING GROWTH FACTOR PROTEIN | 2011 |
|
RU2571926C2 |
| PRODUCING 2S CANOLA PROTEIN USING ION EXCHANGE | 2008 |
|
RU2490274C2 |
| ISOLATION AND PURIFICATION OF ANTI-IL-13 ANTIBODIES USING PROTEIN A AFFINITY CHROMATOGRAPHY | 2010 |
|
RU2603055C2 |
| ANTIBODY PURIFICATION METHODS | 2017 |
|
RU2759909C2 |
