FIELD: biotechnology.
SUBSTANCE: method comprises preparation of a protein-antigen extract from the third stage Anisakis simplex larvae, control of its sterility, determination of protein content, production of hyperimmune serum for protein antigenic extract the 3rd stage A.simplex larvae by immunizing rabbits together with complete Freund's adjunct; immunodiffusion reaction (RID) in agar gel with hyperimmune serum obtained and liquid formed during seafood defrosting. Manifestation of a precipitin band between this liquid and the hyperimmune serum for the protein antigenic A.simplex extract indicates presence of larvae antigens in seafood.
EFFECT: method is effective for anisakids antigens detection in liquid obtained during fish muscle defrosting.
2 dwg, 2 ex
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