FIELD: biotechnology.
SUBSTANCE: method includes: reception of plant explants of Agrobacterium tumefaciens straib and their preparation for inoculation; reception of plant explants and their preparation for inoculation; explant inoculation; explant co-cultivation; explants washing from agrobacteria; explant placing on a medium containing selective agents and bacteriostatic antibiotics; selection of the obtained regenerants on the selective media. The said method is characterized by plant explants preparation for inoculation, explants inoculation with agrobacteria and explants washing from agrobacteria are performed in a culture medium containing all components of the medium for co-cultivation, selection and regeneration, with the exception of gelling agents, plant growth regulators, bacteriostatic antibiotics or selective agents.
EFFECT: application of this method prevents osmotic stress in transformed plant cells and increases the frequency of genetic transformation of plants.
2 cl, 3 dwg, 1 tbl, 6 ex
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