FIELD: medicine.
SUBSTANCE: invention discloses a method for determining the fertility of sperm from patients with idiopathic infertility where patients take two sperm aliquots, one aliquot vozdeystuyut nizkointensivnym millimeter wave electromagnetic radiation having a wavelength λ= 7.1 mm, a frequency of f = 42.194 GHz and power density P = 0.1 mWcm-2⋅, Then the sperm is washed from the seminal plasma, receiving the prototype, and the sperm of the second aliquot is washed to obtain a control sample, after spending resuspension of sperm in the samples later in specimens injected reagents Annexation V-FITC and propidium iodide, the samples are incubated, washed and perform fluorescence microscopy by counting the percentage of the test and control samples stained sperm. Violation of sperm fertility is judged by the value of the index of apoptosis.
EFFECT: improving the accuracy of determining the fertility of sperm in patients with idiopathic infertility.
1 tbl, 3 ex
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Authors
Dates
2017-05-26—Published
2016-05-04—Filed