FIELD: biotechnology.
SUBSTANCE: method for recombinant anti-angiogenic Tumastin peptide a - human tumstatin modified fragment [L69K-95] derivative with Ser-Gly-Ala-Met-Gly and Pro-Gly-Pro sequences attached to N- and C-termini, respectively, provides culturing of E. coli BL21 (DE3)/pTEV-TMS producing strain, destruction of cell biomass in the presence of protease inhibitors, cell lysate centrifuging and separation of cell supernatant containing fusion protein, fusion protein chromatographic purification on a column with an anion exchange sorbent, proteolytic cleavage by TEV-protease, chromatographic purification of the anti-angiogenic Tumastin peptide.
EFFECT: invention provides high yield of anti-angiogenic Tumastin peptide.
2 cl, 5 dwg, 3 ex
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