FIELD: biotechnology.
SUBSTANCE: test system for multiplex quantitative analysis of expression of interferon lambda, MxA antiviral protein and interleukin 23 genes based on reverse transcription combined with real-time polymerase chain reaction, including specific primers and their corresponding fluorescent hydrolysable probes, as well as negative and positive control samples.
EFFECT: invention allows detection, identification and quantification of transcription levels of these genes RNA with high sensitivity, specificity and possibility of multiplex analysis, allows to reduce the cost of analysis by reducing labor, consumption of reagents and plastic and speed up the procedure; can be successfully applied in the control of treatment and prediction the course of human infectious, allergic and autoimmune diseases.
2 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| TEST SYSTEM FOR QUANTITATIVE DIAGNOSTICS OF HUMAN MRNA OF MXA, OAS1, EIF2AK2 GENES BASED ON PCR | 2022 |
|
RU2811690C1 |
| TEST SYSTEM FOR QUANTITATIVE DIAGNOSIS OF MRNA OF HUMAN INTERFERON TYPE I, II AND III BASED ON PCR | 2020 |
|
RU2751791C1 |
| TEST SYSTEM FOR QUANTITATIVE DIAGNOSTICS OF MOUSE OAS1a/g, IL-6, IRF-7, IFNA, IFNB, IFNL GENES BY REAL-TIME PCR | 2022 |
|
RU2796522C1 |
| STRAIN OF ESCHERICHIA COLI BACTERIA - PRODUCER OF IL-29 RECOMBINANT PROTEIN | 2020 |
|
RU2760585C1 |
| MULTIPARAMETRIC DIAGNOSTIC TEST SYSTEM FOR QUANTITATIVE DETERMINATION OF MRNA LEVEL OF HUMAN RIG-1, IFIT-1, IFIH-1 GENES | 2021 |
|
RU2782428C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED DNA PROBES FOR IDENTIFICATION OF RNA OF ENTEROVIRUSES, RHINOVIRUSES, VIRUSES OF HEPATITIS A AND E FROM WATER MEDIUM BY MULTIPLEX PCR METHOD | 2013 |
|
RU2542968C2 |
| RAPID ASSAYS FOR T-CELL ACTIVATION BY RNA MEASUREMENTS USING FLOW CYTOMETRY | 2013 |
|
RU2671707C2 |
| TEST SYSTEM AND METHOD FOR DETECTING RNA OF CORONAVIRUS SARS-COV-2, VIRUS-AGENT OF CORONAVIRUS DISEASE 2019 COVID-19, BY POLYMERASE CHAIN REACTION IN REAL TIME (EMBODIMENTS) | 2020 |
|
RU2731390C1 |
| REAGENT KIT FOR DETECTION OF SARS-COV-2 VIRUS RNA BY REAL-TIMR POLYMERASE CHAIN REACTION | 2022 |
|
RU2795939C2 |
| DIAGNOSTIC TECHNIQUE FOR INFLAMMATORY PROCESS IN EARLY RHEUMATOID ARTHRITIS | 2009 |
|
RU2417263C2 |
