METHOD FOR THE PREPARATION OF SURFACTANT PEPTIDES Russian patent published in 2017 - IPC C07K14/785 C07K19/00 C12P21/02 C12P21/06 C12N15/63 C12N15/70 

FIELD: biotechnology.

SUBSTANCE: expression cassette is constructed containing the sequence encoding the SP-C33(Leu) peptide coding a linker with a protease cleavage site and encoding MBP protein, at that, the said sequences are sequentially fused to a polynucleotide and the polynucleotide is operably linked to a promoter sequence suitable for expression in a prokaryotic cell. The expression cassette is used to prepare an expression vector that is introduced into E. coli cells, cultured under conditions allowing fusion protein expression, purification of the aid protein, followed by fusion protein cleavage with an appropriate protease, and isolation of the SP-C33(Leu) peptide.

EFFECT: increased efficiency of recombinant peptide production.

13 cl, 3 dwg, 1 tbl