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2 628 701

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IPC

C12N15/00(2006-01-01)

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Application

2015153507, 2015-12-14

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Start date

2015-12-14

(22)

Actual filing date

2015-12-14

(45)

Published

2017-08-21

(72)

Inventor

Levitskij Sergej AlekseevichKuzmenko Anton ViktorovichDerbikova Kseniya SergeevnaKrasheninnikov Igor AleksandrovichSamojlova Elena OlegovnaKamenskij Petr Andreevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Gosudarstvennyj Universitet Imeni M.V. Lomonosova"

METHOD TO INCREASE EFFECTIVENESS OF DNA PENETRATION IN EUCARIOTIC CELLS MITOCHONDRIA Russian patent published in 2017 - IPC C12N15/00

Abstract RU 2628701 C2

FIELD: biotechnology.

SUBSTANCE: to implement the method, DNA molecules are pre-treated with recombinant Abf2p protein Saccharomyces cerevisiae, containing mitochondrial signal sequence MNSYSLLTRSFHESSKPLFNLASTLL, at a ratio of 1 protein molecule per 4 binding sites on DNA molecule for 10-30 minutes at 20-30°C in a buffer containing a physiological concentration of NaCl and with pH close to neutral.

EFFECT: invention makes it possible to increase mitochondrial transformation efficiency 8-10 times.

3 dwg, 1 ex

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RU 2 628 701 C2

Authors

Levitskij Sergej Alekseevich

Kuzmenko Anton Viktorovich

Derbikova Kseniya Sergeevna

Krasheninnikov Igor Aleksandrovich

Samojlova Elena Olegovna

Kamenskij Petr Andreevich

Dates

2017-08-21—Published

2015-12-14—Filed