RECOMBINANT PLASMID DNA, A METHOD OF ITS CONSTRUCTION AND KOMAGATAELLA PASTORIS YEAST STRAIN - A PRODUCER OF THE VP2 PROTEIN FRAGMENT OF THE AVIAN INFECTIOUS BURSAL DISEASE VIRUS Russian patent published in 2023 - IPC C12N15/09 C12N15/33 C12N15/81 A61K39/12 

FIELD: biotechnology.

SUBSTANCE: following is proposed: pPICZα-VP2 recombinant plasmid DNA which ensures the synthesis in yeast cells and the secretion of the VP2 protein fragment of the infectious bursal disease virus (IBD) of birds. Also a method of constructing pPICZα-VP2 recombinant plasmid DNA is presented. Komagataella pastoris Y-3489-VP2 recombinant yeast strain is also presented, producing a fragment of the VP2 protein of the avian IBD virus.

EFFECT: invention makes it possible to obtain a fragment of the VP2 protein of the avian IBD virus synthesized and secreted by yeast cells which can be used to create test systems and design subunit vaccines against avian IBD.

3 cl, 5 dwg, 2 ex