METHOD FOR MUSCULAR FIBER TYPES ESTIMATION Russian patent published in 2017 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: to assess the type of muscle fibers, histological sections for determination of oxidative, oxidation-glycolytic and glycolytic types of muscle fibers are successively placed first in a 1% CaCI₂ solution, then in a 2% CoCI₂ solution and further in a 1% ammonium sulfide solution, they are subsequently placed in an acidic medium where the "fast" myosin of glycolytic fibers is inactivated and into an alkaline medium for determination of the oxidation-glycolytic and glycolytic types where the "slow" myosin of oxidative fibers is inactivated. Oxidizing fibers are coloured black on the cut, reddish-glycolytic fibers are gray, and glycolytic fibers are white, the type of muscle fibers is determined by their cross-sectional area, and enzymes are used as markers: succinate dehydrogenase - to evaluate the activity of oxidative processes in mitochondria and lactate dehydrogenase - to determine the intensity of glycolysis in the cell cytoplasm.

EFFECT: method allows to reliably determine the type of muscle fiber when it is available.

3 tbl, 5 dwg