FIELD: biotechnology.
SUBSTANCE: method for vaccine production involves virus antigen cultivation in BHK-21 suspension cell culture at a temperature of 36-37°C, virus suspension purification from ballast admixtures, inactivation, concentration of foot-and-mouth disease virus antigen obtained and antigen concentrate combination with an adjuvant. After 6-8 hours of virus antigen cultivation, percentage of dead cells is registered every 2 hours. When the level of dead cells reaches 95% or more, further cultivation is performed for 2-8 hours depending on the virus type. Foot-and-mouth disease vaccine obtained by this method contains antigen material of A-type and/or O-type and/or Asia-1 type foot-and-mouth disease viruses in an effective quantity of 146S-component, aluminium hydroxide gel, saponin and maintenance medium.
EFFECT: method allows to increase the amount of antigenic material in terms of 146S-component during foot-and-mouth disease virus cultivation, and the vaccine obtained by this method is harmless, avirulent and immunogenic.
13 cl, 1 tbl, 14 ex
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