FIELD: biotechnology.
SUBSTANCE: invention relates to a method for growing a weakened mutant strain of Salmonella typhi carrying a mutation in the galE gene leading to loss of function and containing at least one copy of a recombinant DNA molecule containing a eukaryotic expression cassette. The invention includes the step of cultivating the strain in a buffer medium containing peptone, at about a neutral initial pH value on a fermentation scale in which glucose is not added to the medium during the fermentation process, and the initial amount of glucose is consumed until reaching a stationary phase where the initial pH is from 5 To less than 9, and wherein the volume of the medium is at least about 10 l.
EFFECT: invention can be used for the industrial large-scale production of DNA vaccines based on attenuated strains of Salmonella.
11 cl, 13 dwg, 5 tbl, 6 ex
| Title | Year | Author | Number |
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| DNA-VACCINE FOR APPLICATION IN PATIENTS WITH PANCREATIC CANCER | 2013 |
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| ANTIGEN AND PROTEIN TOXIC CODING NUCLEOTIDE SEQUENCE CARRIER MICROORGANISM | 2007 |
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| METHOD OF PREPARING AN ATTENUATED STRAIN OF BACTERIUM SALMONELLA | 1988 |
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| POLYEPITOPIC ANTI-HEPATITIS B VACCINE OF 4-TH GENERATION AND METHOD OF OBTAINING IT | 2011 |
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|
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| RING DNA MOLECULE WITH CONDITION-DEPENDENT REPLICON ORIGIN, METHOD FOR PRODUCTION THEREOF AND USES IN GENE THERAPY | 2003 |
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| Escherichia coli BACTERIA STRAIN - PRODUCER OF RECOMBINANT FLAGELLIN | 2012 |
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| RECOMBINANT STRAIN OF YEAST Hansenula polymorpha - PRODUCER OF MAJOR CAPSID PROTEIN L1 OF HUMAN PAPILLOMAVIRUS OF TYPE 56 | 2014 |
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