FIELD: biotechnology.
SUBSTANCE: invention relates to a genetic construct for the method for the genetic control of exocytosis. The presented construction has the sequence of SEQ ID NO:4 and is obtained using a lentiviral vector based on HIV-1 modified by sequences encoding a post-transcriptional control element of marmot hepatitis B virus (WPRE), a genetically encoded calcium indicator (GCaMP3) and a cell specific promoter of glial fibrillar acidic protein (GFAP).
EFFECT: invention allows to control exocytosis through specific transfection of astroglial cells and to visualize in vitro transfected cells by embedding the genetically encoded calcium indicator in the developed genetic construct and can be used in medicine, in particular for the study of neurodegenerative diseases.
2 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR NEUROGENESIS CHEMOGENETIC RECORDING AND CORRECTION BASED ON GENETIC CONSTRUCTIONS FOR ASTROCYTES AND NEURONS TRANSFECTION | 2015 |
|
RU2630654C2 |
| METHOD OF GENETIC REGISTRATION AND CORRECTION OF NEUROGENESIS BASED ON GENETIC CONSTRUCTIONS FOR TRANSFECTION OF NEURONS | 2016 |
|
RU2670128C2 |
| GENETIC MAKE-UPS FOR ANTI-HIV THERAPY | 2010 |
|
RU2426788C1 |
| IMPROVING GENETIC CONSTRUCTS FOR PROVIDING HIGH EFFICACY OF ANTI-HIV THERAPY | 2013 |
|
RU2533817C1 |
| GENETIC CONSTRUCT FOR INDUCING PROLIFERATION OF PERIPHERAL MONOCYTES IN VITRO | 2017 |
|
RU2697797C2 |
| METHOD OF SCREENING GAMMA-SECRETASE INHIBITORS | 2004 |
|
RU2373284C2 |
| METHOD OF PRODUCING RECOMBINANT HUMAN GLIAL FIBRILLARY ACIDIC PROTEIN AND THE PRODUCER STRAIN USED IN IT | 2022 |
|
RU2811427C1 |
| EXPRESSIONAL PLASMID LENTIVIRAL VECTOR FOR HETEROLOGICAL EXPRESSION OF RECOMBINANT HUMAN PROTEIN CD47 | 2015 |
|
RU2627181C2 |
| LENTIVIRAL REPORTER VECTOR SYSTEM FOR MEASURING ACTIVITY OF KEY TRANSCRIPTIONAL FACTORS REFLECTING FUNCTIONAL STATUS OF SIGNALLING PATHWAYS OF CELL | 2009 |
|
RU2420584C1 |
| REPORTER SYSTEM BASED ON LENTIVIRUS REPORTER STRUCTURES FOR STUDY OF PROTEIN-PROTEIN INTERACTIONS | 2015 |
|
RU2639539C2 |
