METHOD OF ENVIRONMENTAL MONITORING OF APIARIAN SOILS BY DIATOMIC ALGAE STATE Russian patent published in 2017 - IPC G01N33/24 A01G7/00 B09C1/00 

FIELD: ecology.

SUBSTANCE: method involves selection of point soil samples according to the "wind rose", carried out layer by layer, every 50 cm, to a depth of up to 150 cm, in apiaries located in the industrial zone and in the background areas without industrial emissions of environmental toxicants. During the method, standard reference samples of soil diatoms are obtained, simulating the process of interaction of the diatoms of the background area soils with industrial emissions (pesticides, heavy metals, oil and oil products) under laboratory conditions. Soil samples are prepared by purifying diatom algae contained in the sample from impurities by filtration, washing with acid followed by boiling, rinsing with acid with distilled water, settling and filtration. Diatomaceous preparations are obtained by fixing the diatoms shells in the Kolbe resin, specific identification of diatom algae taxa is performed and the abundance of diatom algae is assessed, and then the state of diatoms in the test plot (presumably contaminated) is compared to the control one (background soil state).

EFFECT: increased accuracy of the determination of pollutants and the level of their concentration in the apiary soil.

6 cl, 1 tbl, 1 ex