FIELD: medicine, pharmacy.
SUBSTANCE: group of invention objects refers to the field of biotechnology. An activated antibody is proposed, comprising a) an antibody or antigen binding fragment (AB), binding to the epidermal growth factor receptor (EGFR); b) masking part (MM), which inhibits the specific AB binding with EGFR in the unsplit state, but does not prevent its binding or compete for binding to EGFR in the split state; c) a split part (CM), which acts as a substrate for the protease; and d) linkers (L1 and L2). The activated antibody in the unsplit state has a structure from N-terminus to C-terminus (MM)-L1-(CM)-L2-(AB) and is activated in the target tissue,localized together with the target and protease for CM. A composition for treatment of tumors, autoimmune diseases or inflammatory diseases or for angiogenesis inhibition; application of the activated antibody for medicament preparation; methods for activated antibody preparation and the isolated nucleic acid molecule comprising the nucleotide sequence of the activated antibody are also described.
EFFECT: invention provides more efficient delivery of activated antibodies to the target cells and may find further application in medicine.
30 cl, 36 dwg, 60 tbl, 19 ex
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