METHOD AND DEVICE FOR INTEGRATING NEURONAL CELLS INTO FORMED NEURAL NETWORK WITH DIRECTED CONNECTIVITY IN VITRO Russian patent published in 2024 - IPC C12M3/00 C12N5/02 C12N5/07 C12N5/71 C12N5/79 

FIELD: biotechnology.

SUBSTANCE: disclosed is a device for growing dissociated neuronal cells based on microfluidic chips combined with microelectrode arrays (MEA), allowing to integrate dissociated neuronal cells into a formed neural network consisting of separate cell populations connected by a weak unidirectional connection. Device comprises three isolated chambers of microfluidic chip, connected by microchannels of asymmetric shape, wherein the height of microchannels is 5-7 mcm, the channels connecting the source chamber and the integration chamber have length of 400 mcm and enable the growth of axons of the source population into the integration chamber for 5 days.

EFFECT: invention provides high accuracy of cell integration into the area of weak directional communication between two subnetworks of neuronal cells, as well as the device is compact and compatible with standard commercial microelectrode arrays, which enables non-invasive evaluation of electrophysiological characteristics of each module.

1 cl, 6 dwg