FIELD: medicine.
SUBSTANCE: method for obtaining of a two-component preparation for treatment of joint damage by low-invasive insertion of the said preparation into the joint bag, in the form of two components, the main and initiating, consisting in the fact that, in order to obtain the main component of the preparation, the blood plasma is transferred with a syringe and a transient filter with a pore size sufficient to ensure sterility, to one of the departments of the cryopack and kept until complete freezing, then the frozen blood plasma is partly defrosted, the first thawed plasma fraction is transferred to the empty compartment of the cryopack and removed from the cryopack by a syringe connected to the pack port, and the cryoprecipitate of the blood plasma remaining in the cryopack is thawed, then the cells which are chondrocyte precursors, are resuspended in the cryoprecipitate of the blood plasma in an amount sufficient to provide a therapeutic effect, and to make the initiating component of the preparation, solution of calcium chloride and thrombin is mixed in a balanced saline solution with addition of aminocaproic acid in an amount not exceeding the maximum recommended dose for a single administration in the joint bag, under certain conditions. A two-component preparation for treatment of joint damage.
EFFECT: method allows for cells localization at the site of administration, allowing the cells to migrate from the glue to the damage site and maintain functional activity.
8 cl, 5 dwg
