FIELD: biotechnology.
SUBSTANCE: invention relates to a method for detection of human or humanized antibodies comprising the steps of treatment of a human or humanized biological sample with a digestible enzyme to form a sample of a split antibody, the biological sample being serum, blood plasma, tissue or cells from an animal that has been treated with a human or humanized antibody; and analysis of a sample of the digested antibody using mass spectrometry to identify one or more human framework peptides.
EFFECT: invention provides detection of therapeutic antibodies in samples from an animal treated with a therapeutic antibody, while other antibodies present in the sample, such as native animal antibodies, are not detected.
12 cl, 4 ex, 25 dwg, 13 tbl
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