FIELD: biotechnology.
SUBSTANCE: present invention relates to the field of biotechnology, specifically to obtaining long-acting insulin analogues, and can be used in medicine for the treatment of diabetes. Insulin analogue is obtained, in which the insulin amino acid is modified by replacing the 14th amino acid of the A-chain with glutamic acid or asparagine, and where the amino acid sequences of the B-chain and A-chain of insulin consist of SEQ ID NO: 38 and 37 respectively. Based on said insulin analogue, a conjugate with a biocompatible substance was obtained as a carrier capable of prolonging the half-life of the insulin analogue in vivo.
EFFECT: invention makes it possible to obtain an insulin analogue having a reduced binding affinity for the insulin receptor compared to the native form.
21 cl, 6 dwg, 5 tbl, 11 ex
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