FIELD: biotechnology.
SUBSTANCE: present invention relates to the field of biotechnology, specifically to a method for producing a conjugate of a physiologically active polypeptide with an immunoglobulin constant region. More specifically, the present invention relates to a method for efficiently producing a conjugate of a physiologically active polypeptide by solving the problem of low yield by using salt in a coupling reaction in preparing a conjugate of a physiologically active polypeptide. Physiologically active polypeptide – non-peptidyl polymer – immunoglobulin constant region conjugate can be obtained in high yield and purity using the production method of the present invention. In addition, the conjugate of a physiologically active polypeptide obtained using this method can be effectively used in the development of a long-acting composition of a physiologically active polypeptide.
EFFECT: improved method for producing a conjugate of a physiologically active polypeptide in high yield is disclosed.
27 cl, 2 tbl, 1 dwg, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| IMPROVED METHOD FOR PHYSIOLOGICALLY ACTIVE POLYPEPTIDE CONJUGATE PRODUCTION | 2013 |
|
RU2639256C2 |
| LIQUID FORMULATION OF LONG-ACTING INSULIN CONJUGATE | 2013 |
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| METHOD FOR PRODUCING PHYSIOLOGICALLY ACTIVE POLYPEPTIDE COMPLEX | 2012 |
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RU2624129C2 |
| LIQUID COMPOSITION OF LONG-ACTING INSULIN AND INSULINOTROPIC PEPTIDE | 2013 |
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| NOVEL INSULIN ANALOGUE AND USE THEREOF | 2014 |
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|
RU2805873C2 |
| METHOD OF CONTROLLING CONDITIONS FOR SITE-SPECIFIC BINDING OF POLYPEPTIDE AND NON-PEPTIDE POLYMER | 2010 |
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RU2495881C2 |
| SUSTAINED TYPE HUMAN GROWTH HORMONE PREPARATION | 2014 |
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RU2683823C2 |
| INSULIN CONJUGATE WITH APPLICATION OF IMMUNOGLOBULIN FRAGMENT | 2011 |
|
RU2519073C1 |
