METHOD FOR MANUFACTURING SENSORS FOR DETERMINATION OF CONCENTRATION OF SUGARS AND HYDROXY ACIDS Russian patent published in 2017 - IPC G01N33/02 

FIELD: medicine.

SUBSTANCE: group of inventions refers to the diagnostic method for determining the concentration of sugars and hydroxy acids by increasing the conductivity of the polymer layer on the surface of the electrode when reacting with the mentioned structures, and can be used to analyze biomolecules, as well as cells which contain structural fragments of sugars or hydroxy acids. For this purpose, a polymer sensor layer is synthesized by electrochemical polymerization of aminophenylboronic acids on the electrode surface. The obtained coating is a conductive substituted polyaniline, characterized by the ability to increase conductivity as a result of the reaction of functional substituents (boronic acid groups) in the polymer with hydroxy acids and sugars. The determination is carried out in an electrochemical cell using a chemical sensor, i.e. an electrode modified with a conductive polyaminophenylboronic acid. An increase in the conductivity of the polymer coating on the surface of the electrode in the presence of the analyzed sample is a signal which is recorded by electrochemical impedance spectroscopy. The quantitative content of the desired component is determined by a calibration curve.

EFFECT: provides accurate determination of the concentration of diols, polyols, mono- and polysaccharides, hydroxy acids and glycosylated biomolecules in model solutions, physiological liquids, medical preparations and food objects.

5 cl, 3 tbl, 3 dwg, 4 ex