ELECTROCHEMICAL SENSOR FOR DETERMINATION OF DOXORUBICIN AND METHOD OF ITS PRODUCTION Russian patent published in 2025 - IPC G01N27/30 

FIELD: chemistry.

SUBSTANCE: invention relates to analytical chemistry and can be used for quantitative determination of the anthracycline preparation of doxorubicin in medicinal preparations, biological preparations and human biological fluids. Electrochemical sensor for determining doxorubicin comprises a planar graphite electrode based on a polyester transparent film of irregular shape, which comprises round part to apply round drop and rectangular part to be connected to potentiostat-galvanostat. Conducting silver paths from polymer silver paste are applied on the base of transparent polyester film along the whole length with ledge by 20 mm. In the centre of the round part of the base made of a transparent polyester film there is a working electrode of graphite-carbon paste in the form of a circle with diameter of 2.2 mm. Auxiliary electrode of carbon-graphite paste in the form of a semicircle and a pseudo-electrode of comparison made of polymer paste Ag/AgCl in the form of a strip are made at the edges of the round part of the base made of transparent polyester film. Conducting silver tracks from polymer silver paste, working electrode from carbon-graphite paste, auxiliary electrode from carbon-graphite paste and pseudo-electrode of comparison from polymer paste Ag/AgCl are fixed on surface of base from polyester transparent film by insulating layer from blue dielectric paste with leaving part of conducting silver tracks from polymer silver paste not coated with insulating layer from blue dielectric paste for possibility of their connection to potentiostat-galvanostat. On the surface of the working electrode from carbon-graphite paste is applied and dried at 100 °C dispersion of carbon black in propylene carbonate. On top of dispersion of carbon black in propylene carbonate there is drop of 10 mcl, containing 0.01 M phosphate buffer solution consisting of 0.01 M disubstituted potassium phosphate trihydrate, 0.1 M potassium chloride and 50 vol.% ethanol, pH=7.0, 0.5-1 mg/ml of DNA from fish milt and 0.07-0.15 mM 3,7-bis(phenylamino)phenothiazine-5-ii chloride.

EFFECT: reduced detection limit and duration of analysis, wider range of determined concentrations, simplified design of sensor and sensor manufacturing procedure.

2 cl, 2 dwg