FIELD: biotechnology.
SUBSTANCE: group of inventions refers to a recombinant microorganism Lactobacillus sp., producing D-lactic acid, a method for its preparation and a method for D-lactic acid production using the said microorganism. Recombinant microorganism Lactobacillus sp. is obtained by inactivation of L-lactate dehydrogenase (L-LDH) and enhancement of D-lactate dehydrogenase (D-LDH) activity in the microorganism Lactobacillus sp., producing more L-lactic acid than D-lactic acid. The said microorganism Lactobacillus sp. is selected from the group consisting of Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus. The method for D-lactic acid production comprises culturing of the said recombinant microorganism Lactobacillus sp. to obtain a culture broth and D-lactic acid extraction from the culture broth.
EFFECT: high yield of D-lactic acid.
13 cl, 1 dwg, 3 tbl, 5 ex
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