FIELD: biotechnology.
SUBSTANCE: invention represents cultural medium for human mesenchymal cells, including basal medium for mesenchymal stem cell, outer layer of white cells lysate/human platelets (buffy coat) from 5 to 20% (vol/vol); insulin from 2 from 20 mg/l; sodium selenite in quantities ranging from 0.005 to 1.730 mg/l; ethanolamine in quantities ranging from 1 to 8 mg/l and basic fibroblast growth factor (bFGF) in quantity from 5 to 25 ng/ml. Mentioned medium is supplemented with fetal bovine serum (FBS) from 10% to 20% (vol/vol) or additive cell culture derived from human plasma (CCS) from 10 to 40% (vol/vol).
EFFECT: invention allows you to cultivate human mesenchymal cells lines, including those which do not grow up in a culture media, that typically use for the cells of this type.
7 cl, 2 tbl, 2 ex
