APPLICATION OF ENDOGENIC DNAASE ACTIVITY TO REDUCE DNA CONTENT Russian patent published in 2018 - IPC C12N1/08 

FIELD: biotechnology.

SUBSTANCE: method includes increase of the pH and/or temperature of the broth in which fungal host cells are cultured for at least 24 hours to a pH of 5.0-9.0 and a temperature of 30-70°C, respectively, incubating the broth at the said elevated pH and/or temperature for a period sufficient to detect a decrease in the host DNA content in the broth. At that, a decrease in the DNA content is not primarily due to the presence of exogenous DNAase in the broth. The method version further includes a step of liquid and solid phases separation to separate the broth from the host cells that are filamentous fungal cells. A method is also proposed for DNA content reduction in a protein preparation obtained from the said host cells. The method includes evaluation of the DNA level of the said host cells in a protein preparation obtained from fungal host cells, increase the pH and/or temperature of the protein preparation, determination of the reduction in the amount of DNA of these host cells in the protein preparation after incubation.

EFFECT: invention effectively reduces DNA in the fermentive broth.

24 cl, 4 dwg, 2 ex